Dolomite develops novel droplet system for encapsulation of single cells or beads

The system represents a major breakthrough in microfluidics by providing users with the tools required to perform assays on individual cells in picolitre volume environments. This novel technology was demonstrated by feeding 2.8µm diameter magnetic beads into monodisperse 100µm diameter droplets at a ratio of 1 bead per droplet with minimal bead losses.

Featuring Dolomite’s standard microfluidic components, the new system allows users to load single cells or beads into droplets. The Mitos P-Pump allows stirring of the input fluid reservoir, to provide an even distribution of beads and delivers a pulseless and stable flow for excellent control of droplet volumes. Reliable and leak-free connections are made from pump to tube to chip using Dolomite’s Multiflux Linear Connectors.

Droplets are isolated in an inert fluorocarbon carrier stream to prevent crosscontamination and are stabilized with Pico-Surf, a biocompatible surfactant designed specifically for generating and stabilizing pico-droplets.