Dolomite creates unique microfluidics chip for creation of single cell libraries

The Single Cell RNA-Seq Chip – a commercially available version of the prototype PDMS Drop­seq chip described by Macosko et al¹ – is manufactured from glass, offering excellent optical transparency and ensuring high precision. Combined with a very gentle sample loop method – to minimise bead shearing and contamination due to premature cell lysis – it enables rapid, straightforward preparation of single cell cDNA libraries. The chip offers robust and reproducible processing of 1 ml samples in approximately 12 hours, allowing cells to be individually encapsulated with uniquely barcoded RNA capture beads for high throughput sequencing.